Production of GPCR and GPCR complexes for structure determination

• Recent methodologies have rendered structure determination of GPCR more feasible.
• Interdisciplinary collaboration has promoted the structural biology of GPCR.
• Growing number of GPCR structures in different states has become available.
• The structure of an active GPCR and G-protein complex has been determined.

Since the first high-resolution structure of the beta 2 adrenergic receptor (b2AR) in 2007, we have seen a growing number of G-protein-coupled receptor (GPCR) structures coming to the repertory, providing a significant progress in our understanding of the structural basis of their function. This has been achieved by the interdisciplinary collaborative work between scientists with various expertise and the development of new methodologies as well as combining and optimizing existing techniques.