Topological constraints: using RNA secondary structure to model 3D conformation, folding pathways, and dynamic adaptation

► Secondary structures can define RNA global architecture and dynamic adaptability. ► Tertiary interactions select for specific conformers from structural ensembles. ► New structure prediction approaches are utilizing topological derived constraints. ► Topological constraints rationalize and describe RNA dynamics and adaptation. ► Topological constraints may direct RNA down specific folding pathways.