Moving DNA around: DNA transposition and retroviral integration

► The DDE family of recombinases includes many DNA transposases, retroviral integrases, and some enzymes that have been domesticated by the host. ► Recent advances in the structural biology of DDE enzymes show common themes despite their highly variable architecture. ► Recent structural studies of Y1 transposases illustrate a completely different pathway for DNA transposition, which utilizes single-stranded DNA substrates.