کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1981288 1061917 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
RecBC enzyme overproduction affects UV and gamma radiation survival of Deinococcus radiodurans
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
RecBC enzyme overproduction affects UV and gamma radiation survival of Deinococcus radiodurans
چکیده انگلیسی

Deinococcus radiodurans recovering from the effect of acute dose of gamma (γ) radiation shows a biphasic mechanism of DNA double strands breaks repair that involves an efficient homologous recombination. However, it shows higher sensitivity to near-UV (NUV) than Escherichia coli and lacks RecBC, a DNA strand break (DSB) repair enzyme in some bacteria. Recombinant Deinococcus expressing the recBC genes of E. coli showed nearly three-fold improvements in near-UV tolerance and nearly 2 log cycle reductions in wild type γ radiation resistance. RecBC over expression effect on radiation response of D. radiodurans was independent of indigenous RecD. Loss of γ radiation tolerance was attributed to the enhanced rate of in vivo degradation of radiation damaged DNA and delayed kinetics of DSB repair during post-irradiation recovery. RecBC expressing cells of Deinococcus showed wild type response to Far-UV. These results suggest that the overproduction of RecBC competes with the indigenous mechanism of γ radiation damaged DNA repair while it supports near-UV tolerance in D. radiodurans.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 7, Issue 1, 1 January 2008, Pages 40–47
نویسندگان
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