کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1981362 1061924 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Study of involvement of ImuB and DnaE2 in stationary-phase mutagenesis in Pseudomonas putida
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Study of involvement of ImuB and DnaE2 in stationary-phase mutagenesis in Pseudomonas putida
چکیده انگلیسی

Several bacterial species carry in their genomes a so-called “mutagenesis” gene cluster encoding ImuB which is similar to Y-family DNA polymerases, and DnaE2 related to the catalytic subunit DnaE of Pol III. Y-family DNA polymerases are known to be involved in stationary-phase mutagenesis and DnaE2 homologues characterized so far have expressed a mutator phenotype. In this study, we raised a question about the involvement of ImuB and DnaE2 in stationary-phase mutagenesis. Here, we show that Pseudomonas putida ImuB and DnaE2 have antagonistic effects on stationary-phase mutagenesis. ImuB facilitated accumulation of stationary-phase mutants up to two-fold. In contrast to that, DnaE2 had no significant effect on emergence of 1-bp deletion mutants and moreover, it acted as an anti-mutator in accumulation of base substitution mutants in starving bacteria. Similar antagonistic effects of DnaE2 and ImuB on mutagenesis appeared also in UV-mutagenesis study. This data distinguishes the DnaE2 of P. putida from its homologues studied in other organisms.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 6, Issue 6, 1 June 2007, Pages 863–868
نویسندگان
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