کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1981398 1061927 2006 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
γH2AX signalling during sperm chromatin remodelling in the mouse zygote
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
γH2AX signalling during sperm chromatin remodelling in the mouse zygote
چکیده انگلیسی

In the mouse, the paternal post-meiotic chromatin is assumed to be devoid of DNA repair after nuclear elongation and protamine-induced compaction. Hence, DNA lesions induced thereafter will have to be restored upon gamete fusion in the zygote. Misrepair of such lesions often results in chromosome type aberrations at the first cleavage division, suggesting that the repair event takes place prior to S-phase. During this stage of the zygotic cell cycle, the paternal chromatin transits from a protamine- to a nucleosome-based state. We addressed the question whether the canonical signalling pathway to DNA double strand breaks (DSBs), the phosphorylated form of histone H2AX (γH2AX) is active during chromatin restructuring of the male genetic complement in the zygote.Here, we describe the detailed characterization of γH2AX signalling in the early stages of zygotic development up to the appearance of the pronuclei.We have found the γH2AX signalling pathway to be already active during sperm chromatin remodelling after gamete fusion in a dose dependent manner, reflecting the amount of DSBs present in the sperm nucleus after in vivo male irradiation. Using DNA damaging compounds to induce lesions in the early zygote, differences in DSB sensitivity and γH2AX processing between paternal and maternal chromatin were found, suggesting differences in DNA repair capacity between the parental chromatin sets.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 5, Issue 8, 13 August 2006, Pages 959–971
نویسندگان
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