کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1981705 1539420 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biophysical analysis of the interaction of the serum protein human β2GPI with bacterial lipopolysaccharide
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Biophysical analysis of the interaction of the serum protein human β2GPI with bacterial lipopolysaccharide
چکیده انگلیسی


• β2-GPI binds more strongly to negatively charged phospatidylserine than to bacterial lipopolysaccharides (LPS).
• β2-GPI has only a moderate tendency to influence LPS-induced cytokine production in vitro.
• β2-GPI reacts exothermally with LPS in a non-saturable way.
• β2-GPI changes its local microenvironment upon LPS association.
• The serum protein β2-GPI is an immune-modulating compound.

There are several human serum proteins for which no clear role is yet known. Among these is the abundant serum protein beta2-glycoprotein-I (β2GPI), which is known to bind to negatively charged phospholipids as well as to bacterial lipopolysaccharides (LPS), and was therefore proposed to play a role in the immune response. To understand the details of these interactions, a biophysical analysis of the binding of β2GPI to LPS and phosphatidylserine (PS) was performed. The data indicate only a moderate tendency of the protein (1) to influence the LPS-induced cytokine production in vitro, (2) to react exothermally with LPS in a non-saturable way, and (3) to change its local microenvironment upon LPS association. Additionally, we found that the protein binds more strongly to phosphatidylserine (PS) than to LPS. Furthermore, β2GPI converts the LPS bilayer aggregates into a stronger multilamellar form, and reduces the fluidity of the hydrocarbon moiety of LPS due to a rigidification of the acyl chains. From these data it can be concluded that β2GPI plays a role as an immune-modulating agent, but there is much less evidence for a role in immune defense against bacterial toxins such as LPS.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Open Bio - Volume 4, 2014, Pages 432–440
نویسندگان
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