کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1982040 1539493 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Epoxide hydrolase activities and epoxy fatty acids in the mosquito Culex quinquefasciatus
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش حشره شناسی
پیش نمایش صفحه اول مقاله
Epoxide hydrolase activities and epoxy fatty acids in the mosquito Culex quinquefasciatus
چکیده انگلیسی


• Multiple EH activities were characterized in the mosquito Culex quinquefasciatus.
• Epoxy fatty acids are endogenous and xenobiotic substrates for EHs from mosquitoes.
• AUDA is a useful inhibitor to investigate the biological roles of epoxy fatty acids.

Culex mosquitoes have emerged as important model organisms for mosquito biology, and are disease vectors for multiple mosquito-borne pathogens, including West Nile virus. We characterized epoxide hydrolase activities in the mosquito Culex quinquefasciatus, which suggested multiple forms of epoxide hydrolases were present. We found EH activities on epoxy eicosatrienoic acids (EETs). EETs and other eicosanoids are well-established lipid signaling molecules in vertebrates. We showed EETs can be synthesized in vitro from arachidonic acids by mosquito lysate, and EETs were also detected in vivo both in larvae and adult mosquitoes by LC-MS/MS. The EH activities on EETs can be induced by blood feeding, and the highest activity was observed in the midgut of female mosquitoes. The enzyme activities on EETs can be inhibited by urea-based inhibitors designed for mammalian soluble epoxide hydrolases (sEH). The sEH inhibitors have been shown to play diverse biological roles in mammalian systems, and they can be useful tools to study the function of EETs in mosquitoes. Besides juvenile hormone metabolism and detoxification, insect epoxide hydrolases may also play a role in regulating lipid signaling molecules, such as EETs and other epoxy fatty acids, synthesized in vivo or obtained from blood feeding by female mosquitoes.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Insect Biochemistry and Molecular Biology - Volume 59, April 2015, Pages 41–49
نویسندگان
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