کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1982419 | 1062286 | 2011 | 6 صفحه PDF | دانلود رایگان |
In the silkworm Bombyx mori and other insects, prothoracicotropic hormone (PTTH) plays a central role in controlling molting and metamorphosis by stimulating the prothoracic glands to synthesize and release the molting hormone ecdysone. Using an AcNPV (Autographa californica nucleopolyhedrovirus)-mediated transient gene transfer system, we identified a cis-regulatory element that participates in the decision to switch expression of PTTH on or off in PTTH-producing neurosecretory cells (PTPCs). The nucleotide sequence of this cis-regulatory element is similar to a cis-regulatory element that participates in direction of expression of diapause hormone-pheromone biosynthesis activating neuropeptide gene (DH-PBAN) ( Shiomi et al., 2007). Furthermore, we found that B. mori Pitx (BmPitx), a bicoid-like homeobox transcription factor, binds the element and activates PTTH expression. Therefore, we propose that the cell-specific expression of two neuropeptide hormone genes, PTTH and DH-PBAN, is activated by the Pitx transcription factor, which may act as a pan-activator in the insect neuroendocrine system and in vertebrate pituitary cells.
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► Identification of a cis-regulatory element that directs prothoracicotropic hormone (PTTH) gene expression using a high-throughput baculovirus-based reporter gene system, the AcNPV gene transfer system.
► Bombyx mori Pitx (BmPitx), a bicoid-like homeobox transcription factor, binds the cis-regulatory element and activates PTTH expression.
► The cell-specific expression of two neuropeptide hormone genes, PTTH and DH-PBAN ( Shiomi et al., 2007), is activated by the Pitx transcription factor, which may act as a pan-activator in the insect neuroendocrine system and in vertebrate pituitary cells.
Journal: Insect Biochemistry and Molecular Biology - Volume 41, Issue 6, June 2011, Pages 356–361