کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1982517 | 1062294 | 2010 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Serotonin regulates an acyl-CoA-binding protein (ACBP) gene expression in the midgut of Rhodnius prolixus Serotonin regulates an acyl-CoA-binding protein (ACBP) gene expression in the midgut of Rhodnius prolixus](/preview/png/1982517.png)
Acyl-CoA esters have many intracellular functions, acting as energy source, substrate for metabolic processes and taking part in cell signaling. The acyl-CoA-binding protein (ACBP), a highly conserved 10 kDa intracellular protein, binds long- and medium-chain acyl-CoA esters with very high affinity, directing them to specific metabolic routes and protecting them from hydrolysis. An ACBP gene sequence was identified in the genome of Rhodnius prolixus. This ACBP gene (RpACBP-1) was expressed in all analyzed tissues and quantitative PCR showed that expression was highest in posterior midgut. In this tissue, ACBP gene expression increased in the first day after blood meal (∼10-fold) and then decreased to unfed levels in the seventh day after meal. Injection of serotonin (5-hydroxytryptamine; 5-HT), a neuroamine released in the hemolymph after the start of feeding, increased the expression of this gene in the midgut of unfed females, reaching levels similar to those observed in fed insects. This effect of injected 5-HT was inhibited by spiperone, an antagonist of 5-HT mammalian receptors, that was also able to block the physiological increase in RpACBP-1 expression observed after feeding. Injection of cholera toxin or dibutyryl-cAMP also resulted in the stimulation of this gene expression. These data reveal a transcriptional regulatory mechanism in R. prolixus, that is triggered by 5-HT. In this way, a novel role for 5-HT is proposed, as a regulator of ACBP gene expression and, consequently, taking part in the control of lipid metabolism.
Journal: Insect Biochemistry and Molecular Biology - Volume 40, Issue 2, February 2010, Pages 119–125