کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1984018 1539927 2011 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Microtubule and kinesin/dynein-dependent, bi-directional transport of autolysosomes in neurites of PC12 cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Microtubule and kinesin/dynein-dependent, bi-directional transport of autolysosomes in neurites of PC12 cells
چکیده انگلیسی

Autophagy, a major degradative pathway of the lysosomal system, has been implicated in various neurodegenerative diseases. During autophagic process, organelles and proteins are encapsulated in double-membrane vacuoles called autophagosomes, which finally fuse with lysosomes to form autolysosomes where incorporated materials are degraded. Despite extensive investigations in identifying the molecular components that participate in autophagy, little is known about routes and dynamics of autophagosomes/autolysosomes in the neurites of live cells. Hence, in the present study, we aim to investigate the biophysical characteristics of neuritic transport of autolysosomes in PC12 cells. Our study demonstrated that monomeric red fluorescence protein-light chain 3 (mRFP-LC3)-labeled autolysosomes were motile and moved along PC12 neurites in both anterograde and retrograde directions with a bias towards the nucleus during starvation. By using image processing, quantitative analysis was made to show the dynamic biophysical characteristics of these vesicles. The average velocity of anterograde and retrograde transport was 0.33 ± 0.04 μm/s and 0.39 ± 0.05 μm/s, respectively. Disruption of microtubules by nocodazole completely abolished their movements, suggesting the neuritic transport of autolysosomes depends on microtubules. The directional transport of autolysosomes was also affected by blockage of motor protein activity. Altogether, our study documents many aspects of the highly dynamic movement of autolysosome in PC12 neurites. Autolysosomes transported in a bi-directional manner along microtubules by dynein and kinesin motor proteins. These findings provide valuable insight into understanding the mechanism and control of autophagy in neurites under physiological and pathological conditions.

Figure optionsDownload high-quality image (191 K)Download as PowerPoint slideHighlights
► We show the bi-directional transport of autolysosomes in PC12 neurites.
► We examine the dynamic biophysical characteristics of these vesicles.
► We found the neuritic directional transport of autolysosomes depends on microtubule.
► Their transports were regulated by molecular motor kinesin and dynein.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 43, Issue 8, August 2011, Pages 1147–1156
نویسندگان
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