کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1984357 | 1539981 | 2006 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The γ subunit of Na+, K+-ATPase: Role on ATPase activity and regulatory phosphorylation by PKA
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In kidney, Na+, K+-ATPase is an oligomer (αβγ) with equimolar amounts of essential α and β subunits and one small hydrophobic FXYD protein (γ subunit). This report describes γ subunit as an activator of pig kidney outer medulla Na+, K+-ATPase in aqueous medium. The effects of γ subunit on Na+, K+-ATPase were dose-dependent and preincubation-dependent. Changes in αβ/γ stoichiometry did not alter Km1 for ATP, and slightly increased Km2, but Vmax was increased at both catalytic and regulatory sites. Hydroxylamine treatment of enzyme phosphorylated by ATP (E-P), in the presence of additional γ subunit, revealed that 52% of the E-P accumulation was not via acyl-phosphate formation. The γ subunit was phosphorylated by endogenous kinases and by commercial catalytic subunit of protein kinase A (PKA). Additionally, we demonstrated that PKA phosphorylation of γ subunit increased its capacity to stimulate ATP hydrolysis. These results suggest that γ subunit can act as an intrinsic Na+, K+-ATPase regulator in kidney.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 38, Issue 11, 2006, Pages 1901-1913
Journal: The International Journal of Biochemistry & Cell Biology - Volume 38, Issue 11, 2006, Pages 1901-1913
نویسندگان
Vanessa Faria Cortes, Fabio Eduardo Veiga-Lopes, Hector Barrabin, Marcelo Alves-Ferreira, Carlos Frederico Leite Fontes,