کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1985713 1540231 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of chitosan nanoparticles coated with hyaluronic acid for topical ocular delivery of dexamethasone
ترجمه فارسی عنوان
توسعه نانوذرات کیتوزان پوشش داده شده با اسید هیالورونیک برای تحویل موضعی چشم دگزامتازون
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی

The present study involved design of dexamethasone-sodium phosphate (DEX) loaded mucoadhesive chitosan nanoparticles for topical ocular delivery to improve its precorneal retention and corneal permeability. The chitosan-sodium tripolyphosphate nanoparticle (CS-NPs) was developed through ionotropic-gelation technique. The developed CS-NPs were coated with hyaluronic-acid (HA) to make discrete, free-flowing NPs and to improve their mucoadhesive characteristics. The particle-size, zeta-potential and polydispersity-index were determined by Malvern-Zetasizer. The average size of the CS-NPs ranged from 305.25 ± 14.29 nm (without HA-coating and before freeze-drying) to 400.57 ± 15.23 nm (HA-coated and after freeze-drying). Due to the polyanionic nature of HA, reversing of zeta-potentials from +32.55 ± 4.15 to −33.74 ± 3.45 was observed. Polydispersity-indices varied from 0.178 ± 0.067 (before freeze-drying of HA-coated F2) to 0.427 ± 0.028 (after freeze-drying of HA-coated F2). The encapsulation and loading capacity of around 72.95% and 14.51% respectively were found in optimized CS-NPs. In simulated tear fluid 75.84% cumulative amount of released drug was detected and the in-vitro release results suggested the mechanism of drug release was Fickian-diffusion type. The clarity, pH, refractive index, surface tension and viscosity of the suspensions of DEX-CS-NPs were found promising for ocular use. Stability study on nanoparticles revealed no significant changes were observed in particle-size, encapsulation, drug release and physicochemical characteristics at 25 °C for 3-months storage.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 89, August 2016, Pages 127–136
نویسندگان
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