Antioxidant and anti-aging activities of the polysaccharide TLH-3 from Tricholoma lobayense

• The aim of the present work was the evaluation of the antioxidant and anti-aging activities of the polysaccharide TLH-3 from Tricholoma lobayense.
• TLH-3 possessed the significant ability to reduce t-BHP induced oxidative damage on human embryonic lung fibroblasts (HELF) cells in vitro.
• TLH-3 could effectively attenuate the oxidative damage and improve parameters related to aging in vivo.

Polysaccharides from edible fungi usually exhibit many bioactivities. Our previous studies found that polysaccharide TLH-3 extracted from Tricholoma lobayense possessed noticeable antioxidant activity. To further explore its biological activities, the antioxidant and anti-aging activities of TLH-3 were evaluated in vitro and in vivo. The results of antioxidant activity in vitro showed that TLH-3 could enhance the cell viability, reduce the production of reactive oxygen species (ROS) and inhibit oxidative damage induced by tert-butylhydroperoxide (t-BHP) in human embryonic lung fibroblasts (HELF) cells. The anti-aging capability was measured in d-galactose (d-gal)-induced aged mice model, and the experimental data showed that TLH-3 significantly inhibited the formation of malondialdehyde (MDA) and raised the activities of superoxide dismutase (SOD) and catalase (CAT) in mice liver and serum (p < 0.05). This study suggested that TLH-3 possessed apparent antioxidant and anti-aging activities and could be exploited as a potent dietary supplement to attenuate aging and prevent age-related diseases in humans.