کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1985812 1540235 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High molecular weight β-poly(l-malic acid) produced by A. pullulans with Ca2+ added repeated batch culture
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
High molecular weight β-poly(l-malic acid) produced by A. pullulans with Ca2+ added repeated batch culture
چکیده انگلیسی

β-Poly(malic acid) (PMLA) has attracted increasing attentions because of its potential application in medicine and other industries. In this study, the variation of PMLA molecular weight (Mw) in the batch culture and the strategies to enhance PMLA Mw were studied. Adding exogenous Ca2+ (0.1 g/L CaCl2) to the medium caused a significant increase in both PMLA concentration and Mw (11.38% and 26.3%, respectively) when Na2CO3 was used as the neutralizer. The Mw of PMLA during the process of batch culture, which associated with the specific PMLA production per unit cell mass (Yp/x) before glucose was depleted, increased from 12.522KDa to its maximum 18.693KDa and then kept decreasing until the end of the culture. Compared with the results in batch culture, Mw increased by 84.4% (up to 19.51 kDa) with a productivity of 1.1 g h−1 L−1 when the cells were maintained in exponential growth phase during Ca2+ added repeated batch culture. The present work provides an efficient approach to obtain superior quality PMLA product with high Mw.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 85, April 2016, Pages 192–199
نویسندگان
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