کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1985882 1540234 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The optimization of isoamylase processing conditions for the preparation of high-amylose ginkgo starch
ترجمه فارسی عنوان
بهینه سازی شرایط پردازش ایزوآمیلاز برای تهیه نشاسته گینکو بالا آمیلوز
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• Basic information on the physicochemical properties of ginkgo starch will assist industrial application.
• Response surface methodology optimized the preparation of high-amylose starch using isoamylase.
• The morphological and physicochemical characteristics of normal and high-amylose ginkgo starches were compared

A high-amylose starch was prepared from ginkgo by hydrolysis using isoamylase and its structures (morphology and crystallinity) and physicochemical properties (swelling factor, water solubility and gelatinization) were determined. The experiments used response surface methodology to determine the optimum parameters for enzymatic hydrolysis: pH 5.0 at 52 °C for 170 min, using an enzyme dose greater than 100 IU/ml. The experimentally observed maximum yield of ginkgo amylose under these conditions was 74.74% and the blue value was 0.756. The high-amylose ginkgo starch showed an irregular surface and porous inner structure while the native starch granules were oval with a smooth surface. X-ray showed that the high-amylose starch displayed a V-type structure. Because of its high amylose content and different structural characteristics, high-amylose starch exhibited a higher gelatinization peak temperature (109.25 °C) and water solubility, and a lower crystallinity (19.13%), gelatinization enthalpy (63.83 J/g), and swelling power. The present study has indicated that high-amylose starch prepared using isoamylase has unique functional properties, which lays the foundation for the wider application of ginkgo starch.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 86, May 2016, Pages 105–111
نویسندگان
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