کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1986340 1540250 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gellan gum microspheres containing a novel α-amylase from marine Nocardiopsis sp. strain B2 for immobilization
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Gellan gum microspheres containing a novel α-amylase from marine Nocardiopsis sp. strain B2 for immobilization
چکیده انگلیسی

A Nocardiopsis sp. stain B2 with an ability to produce stable α-amylase was isolated from marine sediments. The characterization of microorganism was done by biochemical tests and 16S rDNA sequencing. The α-amylase was purified by gel filtration chromatography by using sephadex G-75. The molecular mass of the amylase was found to be 45 kDa by SDS-PAGE and gel filtration chromatography. The isolated α-amylase was immobilized by ionotropic gelation technique using gellan gum (GG). These microspheres were spherical with average particle size of 375.62 ± 21.76 to 492.54 ± 32.18 μm. The entrapment efficiency of these α-amylase loaded GG microspheres was found 74.76 ± 1.32 to 87.64 ± 1.52%. Characterization of α-amylase–gellan gum microspheres was confirmed using FTIR and SEM analysis. The in vitro amylase release kinetic have been studied by various mathematical models that follow the Korsmeyer–Peppas model (R2 = 0.9804–0.9831) with anomalous (non-Fickian) diffusion release mechanism.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 70, September 2014, Pages 292–299
نویسندگان
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