کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1986597 1540256 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Induction of amyloidogenicity in wild type HEWL by a dialdehyde: Analysis involving multi dimensional approach
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Induction of amyloidogenicity in wild type HEWL by a dialdehyde: Analysis involving multi dimensional approach
چکیده انگلیسی

Physiological conditions corresponding to oxidative stress deplete the level of enzyme glyoxalase, facilitating a hike in the serum concentration of glyoxal. Simulating an elevated in vivo level of glyoxal, we tested (50%, v/v) concentration of glyoxal to interact with HEWL. Initially, docking study revealed that glyoxal binds in the hydrophobic core of the enzyme. The interaction between the dialdehyde (glyoxal) and the enzyme (HEWL) followed a three step transition involving pre-molten and molten globule states formed on days 7 and 15 of incubation respectively, which were characterised by an increase in the ANS fluorescence intensity compared to the native state. These molten globule states upon further incubation on day 20 resulted in the formation of aggregates which were characterised by an increase in ThT fluorescence intensity, red shift in Congo red absorbance, negative ellipticity peak at 217 nm in the far-UV CD and the loss of signals at 284, 290 and 294 nm in the near-UV CD spectra. Finally, TEM confirmed the authenticity of lysozyme fibril formation by displaying rod like fibrillar structure.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 64, March 2014, Pages 36–44
نویسندگان
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