کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1987104 | 1540273 | 2012 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Investigation into the mechanism of (â)-epigallocatechin-3-gallate-induced precipitation of insulin
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
EGCGEUIA600UDPPrPRP-HPLCTFASEC(−)-Epigallocatechin-3-gallate - (-) - Epigallocatechin-3-gallateUDI - اداره کل اتباع خارجهTrifluoroacetic acid - اسید Trifluoroaceticinsulin - انسولینTem - این استPrecipitation - بارندگیHydrophobic interaction - تعامل هیدروفوبیکMolecular interactions - تعاملات مولکولیMass spectroscopy - طیف سنجی جرمیTransmission electron microscopy - میکروسکوپ الکترونی عبوریIsoelectric point - نقطه ایزوالکتریکProline-rich protein - پرولین غنی پروتئینreversed-phase high performance liquid chromatography - کروماتوگرافی مایع با کارایی بالا با فاز برگشت پذیر
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The molecular interactions between EGCG and insulin were investigated to probe the mechanism of EGCG-induced insulin precipitation. The results indicated that 1-5Â mM EGCG induced insulin into reversible globular precipitates of 185-365Â nm. The formation of precipitates was facilitated at high salt concentration and pH values close to insulin's isoelectric point, indicating that hydrophobic interaction was the main driving force. The precipitation was positively related to insulin concentration, but for EGCG, there was a suitable concentration (2Â mM at 2Â mg/mL of insulin) at which the precipitate content reached maximum. Mass spectroscopy analysis indicated that EGCG formed clusters in the aqueous solution and the clusters correlate with the insulin precipitation. Based on extensive investigation, a physical model was proposed to explain the molecular interactions between EGCG and insulin. Namely, EGCG monomers and clusters first bound to insulin dimers via hydrophobic interaction, leading to the reduction of the thickness of the hydration layer and the partial denaturation of insulin. Then, EGCG clusters acted as bridges to induce the aggregation and precipitation of insulin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 50, Issue 5, 1 June 2012, Pages 1229-1237
Journal: International Journal of Biological Macromolecules - Volume 50, Issue 5, 1 June 2012, Pages 1229-1237
نویسندگان
Shi-Hui Wang, Xiao-Yan Dong, Yan Sun,