کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1987109 1540273 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Formation of chitin-based nanomaterials using a chitin-binding peptide selected by phage-display
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Formation of chitin-based nanomaterials using a chitin-binding peptide selected by phage-display
چکیده انگلیسی

Targeting polymers with peptides is an efficient strategy to functionalize biomaterials. Phage display technology is one of the most powerful techniques for selecting specific peptides for a wide variety of targets. A method to select a chitin-binding peptide from a 12-mer random peptide library was successfully performed against chitin immobilized in wells of microtiter plates. The synthetic chitin binding peptide (ChiBP) could bind to chitin beads and disrupt their structure. This selected peptide was successfully used to immobilize alkaline phosphatase on chitin. In addition, the peptide could induce colloidal chitin in water to form a chitin coat on the surface of plastic tubes. Scanning electron microscopy (SEM) revealed that the peptide could induce colloidal chitin and chitohexaose to form networks when the temperature was raised to 42 °C.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 50, Issue 5, 1 June 2012, Pages 1267–1274
نویسندگان
, , , ,