کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1987127 1540293 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Wide-angle X-ray scattering as a probe for insulin denaturation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Wide-angle X-ray scattering as a probe for insulin denaturation
چکیده انگلیسی

Wide-angle X-ray scattering (WAXS) from lyophilized protein is characterized by the presence of two relatively broad scattering peaks that are linked to protein structure. This work is concerned with the possibility of utilizing these peaks in the probing of the unfolding and breakdown of insulin. Native insulin is subject to thermal denaturation in the presence and in the absence of thiol catalysts. Denatured products are acid-trapped, lyophilized and monitored using WAXS in addition to Fourier transform infrared spectroscopy (FTIR), gel filtration chromatography and Transmission Electron Microscopy (TEM) as supportive techniques. Results show that the WAXS peak at a d-spacing about 10 Å is sensitive towards the α-helix content of insulin. A reduction in the intensity of such peak is proven to be directly linked to the reduction of native insulin having normal α-helix content. The supportive techniques confirmed the decrease in the α-helix content of insulin which accompanied the different denaturation treatments.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 46, Issue 5, 1 June 2010, Pages 471–477
نویسندگان
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