کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1987139 1540293 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A procedure for characterizing glucans synthesized by purified enzymes of cariogenic Streptococcus mutans
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
A procedure for characterizing glucans synthesized by purified enzymes of cariogenic Streptococcus mutans
چکیده انگلیسی

Extracellular polysaccharide is a virulence factor of the most cariogenic bacteria, Streptococcus mutans. We describe a procedure for chemical analysis of this polysaccharide of S. mutans, using a well-known glucan synthesized by purified enzymes of cariogenic streptococci. Soluble and insoluble glucan from glucosyltransferase D and B respectively, were prepared and analyzed by nuclear magnetic resonance spectroscopy and methylation. Soluble α-glucan contained 60% of (1 → 6)-linkages and 17% of branching while insoluble α-glucan was composed of 57% of (1 → 3)- and 28% of (1 → 6)-linkages with 8% branching. The described procedure could be important focusing future studies on in vivo biofilm.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 46, Issue 5, 1 June 2010, Pages 551–554
نویسندگان
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