Optimization of extracellular glucan production from Pleurotus eryngii and its impact on angiogenesis

► Pleurotus eryngii, an edible mushroom optimized using response surface methodology of four-factor Box-Behnken design. ► The optimum levels of glucose, yeast extract, pH and temperature were determined for maximal yield of mycelial biomass and extra cellular polysaccharide. ► The Box Behnken design matrix was validated using real-time experiments. ► Extracellular polysaccharide of Pleurotus eryngii was found to be a glucan. ► Extracellular glucans significantly inhibited the neo-vascularization above 500 μg/egg.