Immobilization of acetylcholinesterase on nanostructure polyacrylonitrile membranes

AChE was covalently immobilized onto nanostructured membranes using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride. The relationship between immobilization factors and enzyme activity were examined by the series of contour plots. The selections of the immobilization variable range were extremely precise in the 3-level-3-factor fractional design. The results indicated that the optimal conditions for AChE immobilization were: 0.025% enzyme solution; immobilization temperature, 4 °C and immobilization time, 15 h. The biochemical characteristics and kinetic parameters of immobilized enzyme were determined.