کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1987811 1540302 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Short neuroprotective peptides, ADNF9 and NAP, are structurally disordered and monomeric in PBS
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Short neuroprotective peptides, ADNF9 and NAP, are structurally disordered and monomeric in PBS
چکیده انگلیسی

Activity-dependent neurotrophic factor 9 (ADNF9) and NAP are nine and eight amino acid peptides, which exhibit neuroprotective activity at femtomolar concentrations against cell toxic agents. We have here characterized their structures and interactions with dodecylphosphocholine (DPC) in phosphate-buffered saline (PBS). Circular dichroism analysis showed that ADNF9 and NAP are structurally disordered in PBS independent of peptide concentration and temperature, but appear to assume different secondary structure at increasing temperature. Sedimentation equilibrium analysis showed that both ADNF9 and NAP are monomeric at 37 °C, suggesting no self-association under physiological conditions. No secondary structure changes were observed in the presence of DPC, suggesting that ADNF9 and NAP do not interact with lipids.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 45, Issue 1, 1 July 2009, Pages 8–11
نویسندگان
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