کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1988475 | 1063307 | 2007 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hydrolytic activity of lipase on anion-exchange solid phase column after separation and electrotransfer by non-denaturing electrophoresis
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کلمات کلیدی
SPE2-DECBBIEFTEMEDQmaCoomassie Brilliant Blue - Coomassie درخشان آبیESI-MS/MS - ESI-MS / MSSolid phase extraction - استخراج فاز جامدtwo-dimensional gel electrophoresis - الکتروفورز ژل دو بعدیisoelectric focusing - تمرکز ذره ای الکتریکیElectrospray ionization tandem mass spectrometry - طیف سنجی جرمی یونیزه الکترو اسپری یونیزاسیونphosphatidylcholine - فسفاتیدیل کولینMALDI-TOF MS - مالدی توف MS
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Hydrolytic activity of lipase on anion-exchange solid phase column after separation and electrotransfer by non-denaturing electrophoresis Hydrolytic activity of lipase on anion-exchange solid phase column after separation and electrotransfer by non-denaturing electrophoresis](/preview/png/1988475.png)
چکیده انگلیسی
This study reports the initial separation of phospholipase C-alpha from porcine retina using non-denaturing two-dimensional gel electrophoresis (2-DE). Detection was by negative staining and then its hydrolytic activity was estimated using α-naphthyl acetate in a 2-DE gel. A spot of phospholipase C-alpha separated by 2-DE was excised. It was then electrophoretically transferred to an anion-exchange solid phase column after 40 mg, equal to dry weight of the solid resin from the cartridge (Accell⢠Plus QMA, Waters Corporation), was packed into a disposable 1 ml syringe to make an anion-exchange solid phase column. Phosphatidylcholine was hydrolyzed in the anion-exchange solid phase column containing phospholipase C-alpha. The results indicated that a column with hydrolytic activity could be produced once lipases separated by non-denaturing 2-DE were transferred to the solid phase column.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biochemical and Biophysical Methods - Volume 70, Issue 3, 10 April 2007, Pages 487-491
Journal: Journal of Biochemical and Biophysical Methods - Volume 70, Issue 3, 10 April 2007, Pages 487-491
نویسندگان
Youji Shimazaki,