کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1988594 | 1063317 | 2007 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: HPLC separation of some purine and pyrimidine derivatives on Chromolith Performance Si monolithic column HPLC separation of some purine and pyrimidine derivatives on Chromolith Performance Si monolithic column](/preview/png/1988594.png)
The chromatographic behavior of some purines and pyrimidines on a monolithic Chromolith Performance Si column under normal-phase high-performance liquid chromatography mode has been studied. Column pressure, column efficiency and selectivity of Chromolith Performance Si column were compared to those of conventional spherical 5 μm silica packed columns Econosphere Silica and Zorbax Rx-SIL. The investigation has shown that application of Chromolith Performance Si column for analysis of polar solutes can reduce the separation time without sacrificing column efficiency and selectivity. Improvement of the monolithic silica column efficiency for polar solutes is observed when ternary mobile phases (mixtures of hexane–isopropanol with ethylene glycol, water or acetonitrile) are applied.
Journal: Journal of Biochemical and Biophysical Methods - Volume 70, Issue 1, 23 February 2007, Pages 15–21