کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1988599 | 1063317 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High-throughput protein digestion by trypsin-immobilized monolithic silica with pipette-tip formula
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: High-throughput protein digestion by trypsin-immobilized monolithic silica with pipette-tip formula High-throughput protein digestion by trypsin-immobilized monolithic silica with pipette-tip formula](/preview/png/1988599.png)
چکیده انگلیسی
Based on the monolithic silica gel materials with hierarchical pore structure and on the SPE devices (MonoTip®) developed thereof, a trypsin-immobilized monolithic silica in a pipette tip (MonoTip® Trypsin) suitable for digesting proteins has been newly developed. The surface of monolithic silica fixed into the tip was chemically modified with trypsin via an aminopropyl group. Trypsin-immobilized monolith successfully performed a rapid digestion of reduced and alkylated proteins with only a few times pipetting operation for the pre-treatment procedure of chromatographic analysis. The novel solid-phase digestion tool using monolithic silica allows a high-throughput trypsin proteolysis of bio-substances in proteomics.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biochemical and Biophysical Methods - Volume 70, Issue 1, 23 February 2007, Pages 57–62
Journal: Journal of Biochemical and Biophysical Methods - Volume 70, Issue 1, 23 February 2007, Pages 57–62
نویسندگان
Shigenori Ota, Shota Miyazaki, Hideo Matsuoka, Kei Morisato, Yukihiro Shintani, Kazuki Nakanishi,