کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1988614 | 1540396 | 2006 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A colorimetric assay for S-adenosylhomocysteine hydrolase
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
A colorimetric method for S-adenosyl-l-homocysteine hydrolase (SAHase) which uses S-adenosyl-l-homocysteine (SAH) as substrate is described. This method involves the hydrolytic conversion of SAH into adenosine (ADO) and l-homocysteine (HCY). The formation of HCY is quantified using Ellman's reagent and spectrophotometrical measured at 412 nm. Under these assay conditions, the product was followed continuously in a facile and quantitative manner until substrate conversion was complete. This method is an easy, cheap and shorter alternative to more complex methods and it is applicable to routine clinical analysis and in the assay and development of new S-nucleosidylhomocysteines to be used as therapeutic compounds.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biochemical and Biophysical Methods - Volume 67, Issues 2–3, 30 June 2006, Pages 131–140
Journal: Journal of Biochemical and Biophysical Methods - Volume 67, Issues 2–3, 30 June 2006, Pages 131–140
نویسندگان
J.D. Lozada-Ramírez, I. Martínez-Martínez, A. Sánchez-Ferrer, F. García-Carmona,