کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1990837 1540708 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Early postprandial low-grade inflammation after high-fat meal in healthy rats: possible involvement of visceral adipose tissue
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Early postprandial low-grade inflammation after high-fat meal in healthy rats: possible involvement of visceral adipose tissue
چکیده انگلیسی

In the postprandial period, low-grade inflammation may contribute to vascular endothelial dysfunction, a hallmark of atherogenesis. Little is known about the involvement of the adipose tissue in the initiation of the postprandial inflammatory response such as obtained after a high-saturated fat meal (HFM). In the present study, we first studied the time course of appearance of systemic inflammation after a HFM in healthy rats, and then we investigated whether a HFM activates the inflammatory signaling in the visceral adipose tissue, with a focus on the key component, nuclear factor-κB (NF-κB).Two hours after the HFM, plasma IL-6 and PAI-1, but not plasma C-reactive protein and soluble intracellular adhesion molecule-1, showed a marked, transient increase. These changes were specific to the postprandial state as not observed after a control water load. Neutrophils count and activation markers CD11B and CD62L, assessed by flow cytometry, also rose significantly 2 h after the HFM, while remaining steady after the control. At the same time, the HFM decreased significantly B-cell count and expression of the activation marker CD62L. Interestingly, at the same early time after the HFM, in the visceral adipose tissue, there was a 2.2-fold increase in the activation of NF-κB (p65) in nuclear extract and an increase in IL-6 mRNA.As far as we know, this is the first study evidencing an acute, postprandial activation of inflammation in visceral adipose tissue. This early activation of NF-κB pathway after a HFM may play a triggering role in the initiation of the complex postprandial proatherogenic phenotype.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Nutritional Biochemistry - Volume 21, Issue 6, June 2010, Pages 550–555
نویسندگان
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