کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1990983 1540761 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Decreased TGF-β1 and IGF-1 protein expression in rat embryo skull bone in folic acid-restricted diet
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Decreased TGF-β1 and IGF-1 protein expression in rat embryo skull bone in folic acid-restricted diet
چکیده انگلیسی
Folic acid deficiency during conception up to the end of the third month of gestation is believed to play the most important factor in neural tube defects (NTDs). However, the exact molecular mechanism remains to be elucidated. It has been suggested that transforming growth factor-β (TGF-β1) and insulin-like growth factor-1 (IGF-1) play a critical role in supporting bone formation. Therefore, folic acid deficiency may contribute to NTD occurrence via decreased TGF-β1 and IGF-1 expression. This study aimed to determine the correlation between folic acid deficiency and the expression of TGF-β1 and IGF-1 in rat skull bone. Thirty female Sprague-Dawley rats were divided into three groups. Purified diet containing 5 (restricted), 15 (low) and 30 μg (normal) of folic acid was given to the first, second and third groups, respectively. At 16 weeks of a given diet, blood samples were taken to examine folic acid (folate immunoassay method), TGF-β1 and IGF-1 (enzyme-linked immunosorbent assay method) levels. After forced mating, on the 18th-19th day of gestation (E18-19), the pregnant rats were subjected to hysterectomy. The skull bone samples of E18-19 rats were taken to examine the TGF-β1 and IGF-1 protein expression by immunohistochemistry. The folic acid-restricted diet (5 μg) resulted in decreased serum TGF-β1 and IGF-1 levels. Furthermore, protein expression of TGF-β1 and IGF-1 in E18-19 rat skull bones was also significantly lower in the folic acid-restricted diet than in the normal diet. Folic acid deficiency could result in reduction of TGF-β1 and IGF-1 protein levels and might contribute to formation of defects in the skull bone as observed in mengingocele patients.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Nutritional Biochemistry - Volume 17, Issue 1, January 2006, Pages 51-56
نویسندگان
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