کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1993424 1064666 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Profiling post-transcriptionally networked mRNA subsets using RIP-Chip and RIP-Seq
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Profiling post-transcriptionally networked mRNA subsets using RIP-Chip and RIP-Seq
چکیده انگلیسی

Post-transcriptional regulation of messenger RNA contributes to numerous aspects of gene expression. The key component to this level of regulation is the interaction of RNA-binding proteins (RBPs) and their associated target mRNA. Splicing, stability, localization, translational efficiency, and alternate codon use are just some of the post-transcriptional processes regulated by RBPs. Central to our understanding of these processes is the need to characterize the network of RBP-mRNA associations and create a map of this functional post-transcriptional regulatory system. Here we provide a detailed methodology for mRNA isolation using RBP immunoprecipitation (RIP) as a primary partitioning approach followed by microarray (Chip) or next generation sequencing (NGS) analysis. We do this by using specific antibodies to target RBPs for the capture of associated RNA cargo. RIP-Chip/Seq has proven to be is a versatile, genomic technique that has been widely used to study endogenous RBP-RNA associations.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 67, Issue 1, 1 May 2014, Pages 13–19
نویسندگان
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