کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1993429 | 1064666 | 2014 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: BRIC-seq: A genome-wide approach for determining RNA stability in mammalian cells BRIC-seq: A genome-wide approach for determining RNA stability in mammalian cells](/preview/png/1993429.png)
• BRIC-seq method is suitable for the determination of genome-wide RNA stability.
• BRIC-seq can determine the RNA half-life of each transcript.
• Protocols for BrU-labelling of RNA, cDNA library construction and data analysis.
We recently developed a novel transcriptome analysis method, termed 5′-bromo-uridine (BrU) immunoprecipitation chase-deep sequencing analysis (BRIC-seq). BRIC-seq enables the determination of genome-wide RNA stability by chasing chronological decreases of BrU-labeled RNAs under physiologically undisturbed conditions. The RNA half-life of each transcript is calculated from the decreasing number of BrU-labeled RNA sequence tags measured by deep sequencing of BrU-labeled RNAs. Here, we describe a detailed protocol and provide tips for BRIC-seq, followed by computational analysis.
Journal: Methods - Volume 67, Issue 1, 1 May 2014, Pages 55–63