کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1993650 1064696 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Phage selection of bicyclic peptides
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Phage selection of bicyclic peptides
چکیده انگلیسی

Bicyclic peptides are small, constrained peptides that can bind with high affinity and selectivity to protein targets. Their small size provides a number of advantages over larger protein-based ligands, including access to chemical synthesis, better tissue penetration, and a wider choice of application routes. Bicyclic peptide ligands can be identified using phage display technology with moderate effort and cost. Here we provide step-by-step protocols for the isolation of bicyclic peptide ligands using phage display. These protocols have been successfully used in our laboratory for the generation of high-affinity binders to a variety of protein targets. We describe library generation, affinity selection and ligand characterization, and provide troubleshooting advice concerning frequent problems.


► Bicyclic peptides can bind with high affinity and selectivity to protein targets.
► They provide an attractive format for the generation of therapeutics.
► We present a step-by-step protocol for the phage selection of bicyclic peptides.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 60, Issue 1, 15 March 2013, Pages 46–54
نویسندگان
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