کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1993650 | 1064696 | 2013 | 9 صفحه PDF | دانلود رایگان |

Bicyclic peptides are small, constrained peptides that can bind with high affinity and selectivity to protein targets. Their small size provides a number of advantages over larger protein-based ligands, including access to chemical synthesis, better tissue penetration, and a wider choice of application routes. Bicyclic peptide ligands can be identified using phage display technology with moderate effort and cost. Here we provide step-by-step protocols for the isolation of bicyclic peptide ligands using phage display. These protocols have been successfully used in our laboratory for the generation of high-affinity binders to a variety of protein targets. We describe library generation, affinity selection and ligand characterization, and provide troubleshooting advice concerning frequent problems.
► Bicyclic peptides can bind with high affinity and selectivity to protein targets.
► They provide an attractive format for the generation of therapeutics.
► We present a step-by-step protocol for the phage selection of bicyclic peptides.
Journal: Methods - Volume 60, Issue 1, 15 March 2013, Pages 46–54