کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1993748 1064703 2012 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The bacterial two-hybrid system based on adenylate cyclase reconstitution in Escherichia coli
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The bacterial two-hybrid system based on adenylate cyclase reconstitution in Escherichia coli
چکیده انگلیسی

The bacterial two-hybrid system based on the reconstitution of adenylate cyclase in Escherichia coli (BACTH) was described 14 years ago (Karimova, Pidoux, Ullmann, and Ladant, 1998, PNAS, 95:5752). For microbiologists, it is a practical and powerful alternative to the use of the widely spread yeast two-hybrid technology for testing protein–protein interactions. In this review, we aim at giving the reader clear and most importantly simple instructions that should break any reticence to try the technique. Yet, we also add recommendations in the use of the system, related to its specificities. Finally, we expose the advantages and disadvantages of the technique, and review its diverse applications in the literature, which should help in deciding if it is the appropriate method to choose for the case at hand.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 58, Issue 4, December 2012, Pages 325–334
نویسندگان
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