کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1993867 1064714 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Large scale expression and purification of recombinant RNA in Escherichia coli
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Large scale expression and purification of recombinant RNA in Escherichia coli
چکیده انگلیسی

Stable, folded RNA are involved in many key cellular processes and can be used as tools for biological, pharmacological and/or molecular design studies. However, their widespread use has been somewhat limited by their fragile nature and by the difficulties associated with their production on a large scale, which were limited to in vitro methods. This work reviews the novel techniques recently developed that allow efficient expression of recombinant RNA in vivo in Escherichia coli. Based on the extensive data available on the genetic and metabolic mechanisms of this model organism, conditions for optimal production can be derived. Combined with a large repertoire of RNA motifs which can be assembled by recombinant DNA techniques, this opens the way to the modular design of RNA molecules with novel properties.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 54, Issue 2, June 2011, Pages 267–273
نویسندگان
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