کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1993892 1064716 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Studies of HIV-1 latency in an ex vivo model that uses primary central memory T cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Studies of HIV-1 latency in an ex vivo model that uses primary central memory T cells
چکیده انگلیسی

HIV-1 latency is considered the last hurdle toward viral eradication in the presence of antiretroviral therapy. Studies of viral latency in vivo are complicated by the low frequency of latently infected cells found in HIV-1 patients. To be able to study the signaling pathways and viral determinants of latency and reactivation, we have developed a novel method that generates high numbers of latently HIV-1 infected cells, which are derived from human primary CD4+ T lymphocytes. This method allows for the study of different aspects of HIV-1 latency, such as the transcription factors needed for viral reactivation and the signaling pathways involved. In this review, we describe in detail an experimental protocol for the generation of HIV-1 latency using human primary CD4+ T cells. We also present the salient points of other latency models in the field, along with key findings arising from each model.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 53, Issue 1, January 2011, Pages 54–61
نویسندگان
, ,