کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1993961 1064721 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Caenorhabditiselegans as a model system for mtDNA replication defects
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Caenorhabditiselegans as a model system for mtDNA replication defects
چکیده انگلیسی

Since the isolation and physical characterization of mammalian mitochondrial DNA (mtDNA) over 35 years ago, numerous studies have been conducted in order to understand its structure and properties, including mode of mtDNA replication and transcription. Even today, the mode of mtDNA replication is still a matter of intense debate. We believe that Caenorhabditis elegans holds the promise of identifying molecular mechanisms of mitochondrial replication. C. elegans is a simple and extremely powerful genetic and developmental model system. Their small size, rapid life cycle, the ability to self-fertilize and somatic tissues that consist of post-mitotic cells offer an efficient way to study mitochondrial metabolism. We have recently developed a number of methods in order to study mitochondrial DNA level and mtDNA maintenance during the development of C. elegans. We hope that the techniques described here can assist laboratories interested in understanding modes of mtDNA replication, distribution and mitochondrial morphology in C. elegans.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 51, Issue 4, August 2010, Pages 437–443
نویسندگان
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