کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1994091 1064734 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Constructing atomic-resolution RNA structural ensembles using MD and motionally decoupled NMR RDCs
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Constructing atomic-resolution RNA structural ensembles using MD and motionally decoupled NMR RDCs
چکیده انگلیسی

A broad structural landscape often needs to be characterized in order to fully understand how regulatory RNAs perform their biological functions at the atomic level. We present a protocol for visualizing thermally accessible RNA conformations at atomic-resolution and with timescales extending up to milliseconds. The protocol combines molecular dynamics (MD) simulations with experimental residual dipolar couplings (RDCs) measured in partially aligned 13C/15N isotopically enriched elongated RNA samples. The structural ensembles generated in this manner provide insights into RNA dynamics and its role in functionally important transitions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 49, Issue 2, October 2009, Pages 167–173
نویسندگان
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