کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1994248 1064757 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro generation of three-dimensional renal structures
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
In vitro generation of three-dimensional renal structures
چکیده انگلیسی

End-stage renal disease is currently being treated effectively by transplantation. However, increasing demand and donor shortage make this treatment challenging. Recent advances in cell-based therapies have provided potential opportunities to alleviate the current challenges of donor shortage. In this study we developed a system to generate renal structures in vitro using primary kidney cells. This system involves the cultivation of expanded primary renal cells in a three-dimensional collagen-based culture system. After one week of growth, individual renal cells began to form renal structures resembling tubules and glomeruli. Histologically, these structures show phenotypic resemblance to native kidney structures. The reconstituted tubules stained positively for Tamm–Horsfall protein, which is expressed in the thick ascending limb of Henle’s Loop and distal convoluted tubules. These results show that renal structures can be reconstituted in a three-dimensional culture system, which may eventually be used for renal cell therapy applications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 47, Issue 2, February 2009, Pages 129–133
نویسندگان
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