کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1994273 1064760 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Light microscopic methods to visualize mitochondria on tissue sections
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Light microscopic methods to visualize mitochondria on tissue sections
چکیده انگلیسی

Mitochondria are cytoplasmic, double-membrane organelles, a main role of which is to synthesize ATP, the universal energy ‘supply’ of cells. In the last three decades, molecular genetic, biochemical, immunological and cell biological techniques have been applied in a coordinated fashion to unveil the pathogenesis of known mitochondrial disorders, as well as to explore the role of mitochondria in aging and neurodegenerative diseases. Once to be thought to be rare, it is now clear that mitochondrial dysfunction is an important cause of neurological and cardiac diseases, and age-related disorders such as cancer. Here, we review, illustrate, and provide updated protocols of two histochemical, and three immunohistochemical methods that in our opinion are the most reliable tools to visualize mitochondria on tissue sections from normal and disease specimens.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 46, Issue 4, December 2008, Pages 274–280
نویسندگان
, ,