کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1994380 1064775 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
[3′-32P]-labeling tRNA with nucleotidyltransferase for assaying aminoacylation and peptide bond formation
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
[3′-32P]-labeling tRNA with nucleotidyltransferase for assaying aminoacylation and peptide bond formation
چکیده انگلیسی

The analysis of reactions involving amino acids esterified to tRNAs traditionally uses radiolabeled amino acids. We describe here an alternative assay involving [3′-32P]-labeled tRNA followed by nuclease digestion and TLC analysis that permits aminoacylation to be monitored in an efficient, quantitative manner while circumventing many of the problems faced when using radiolabeled amino acids. We also describe a similar assay using [3′-32P]-labeled aa-tRNAs to determine the rate of peptide bond formation on the ribosome. This type of assay can also potentially be adapted to study other reactions involving an amino acid or peptide esterified to tRNA.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 44, Issue 2, February 2008, Pages 74–80
نویسندگان
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