کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1994729 1541289 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Role of advanced glycation end product (AGE)-induced receptor (RAGE) expression in diabetic vascular complications
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Role of advanced glycation end product (AGE)-induced receptor (RAGE) expression in diabetic vascular complications
چکیده انگلیسی


• Vascular complications in T2DM is associated with increase AGE level and RAGE expression.
• RAGE expression correlates significantly with serum AGE level in T2DM patients.
• AGE-mediated enhanced RAGE expression contributes to vascular complications.

AimsVascular complications are the major causes of morbidity and mortality in diabetic subjects. Interaction of advanced glycation end products (AGEs) with their receptor (RAGE) induces signal transduction that culminates in vascular complications. Therefore, in the present study we investigated the dependence of RAGE expression on circulating AGEs and evaluated the outcome of AGE–RAGE interaction by the oxidative stress and nature of vascular complications in type 2 diabetes mellitus (T2DM) patients.MethodsRAGE expression was determined by quantitative real-time PCR and western blotting, serum AGEs were estimated by ELISA and spectrofluorometry and oxidative stress markers namely protein carbonyl (PCO), advanced oxidation protein products (AOPP) and lipid peroxidation (MDA) were assayed spectrophotometerically in 75 T2DM patients (DM without vascular complication n = 25; DM with microvascular complications n = 25; DM with macrovascular complications n = 25) and 25 healthy controls.ResultsSerum AGE level was significantly higher in diabetic patients having vascular complications as compared to T2DM without complications (p < 0.01). RAGE m-RNA expression level in PBMCs assayed by quantitative real time PCR was four times higher in diabetic subjects without vascular complications while DM patients having microvascular and macrovascular complications showed 12 fold and 8 fold higher RAGE m-RNA expression respectively compared to healthy controls. Circulating AGE level showed significant positive correlation with RAGE m-RNA expression and oxidative stress markers.ConclusionAGE-mediated exacerbation of RAGE expression may contribute to oxidative stress generation that plays a key role in pathogenesis of vascular complications in diabetes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microvascular Research - Volume 95, September 2014, Pages 1–6
نویسندگان
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