کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1994797 | 1541291 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pericytes prevent regression of endothelial cell tubes by accelerating metabolism of lysophosphatidic acid
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کلمات کلیدی
ATXBRECBRPAutotaxinLPAMMPLPCmonoacylglycerolHUVECAMDHEKMAGLPPTLCphosphatidic acid - اسید فسفاتیدیکlysophosphatidic acid - اسید لیسفسفیدیدTIMP - زمانHuman umbilical vein endothelial cell - سلول اندوتلیالی ورید ناقل انسانیEndothelial cell - سلول های اندوتلیالage-related macular degeneration - سن تخریب ماکولا مربوط به سن استVascular endothelial growth factor A - فاکتور رشد اندوتلیال عروقی ALysophosphatidylcholine - لیزوفسفاتیدیل کولینLipid phosphate phosphatase - لیپید فسفات فسفاتازmatrix metalloproteinase - ماتریکس متالوپروتئینازtissue inhibitor of matrix metalloproteinases - مهار کننده بافت متالوپروتئیناز ماتریکسthin layer chromatography - کروماتوگرافی لایه نازک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Efforts to eradicate pathological vessels in neovascular diseases and induce growth of mature, functional vasculature in ischemic diseases are limited by our incomplete understanding of molecular mechanisms of vessel stabilization. While it is well known that pericytes stabilize blood vessels, the underlying mechanisms have not been fully elucidated. The goal of this study was to further investigate the mechanisms by which pericytes stabilize vessels. In an in vitro model of blood vessels, in which regression is driven by lysophosphatidic acid (LPA), pericyte-mediated stabilization was associated with a decrease in the concentration of LPA. The decline in the concentration of LPA was not caused by a reduction in activity or expression of autotaxin, the main enzyme implicated in LPA production. Rather, pericytes accelerated LPA metabolism. Stabilization of tubes by pericytes correlated with accelerated LPA dephosphorylation and increased expression of lipid phosphate phosphatases (LPPs). Finally, pericytes failed to stabilize tubes exposed to an LPA analogue, which was resistant to degradation. Our results suggest that pericytes stabilize endothelial cell tubes by accelerating the metabolism of LPA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microvascular Research - Volume 93, May 2014, Pages 62-71
Journal: Microvascular Research - Volume 93, May 2014, Pages 62-71
نویسندگان
RÅ«ta MotiejÅ«naitÄ, Jorge Aranda, Andrius Kazlauskas,