Chemical Genetic Screen for AMPKα2 Substrates Uncovers a Network of Proteins Involved in Mitosis

► The energy-sensing kinase AMPK directly phosphorylates proteins involved in mitosis ► The phosphatase regulatory subunit PPP1R12C and the kinase PAK2 are AMPK substrates ► AMPK activity and PPP1R12C phosphorylation are particularly high in mitosis ► Altering AMPK activity or PPP1R12C phosphorylation impairs proper mitotic completion