Nascent Peptide in the Ribosome Exit Tunnel Affects Functional Properties of the A-Site of the Peptidyl Transferase Center

SummaryThe ability to monitor the nascent peptide structure and to respond functionally to specific nascent peptide sequences is a fundamental property of the ribosome. An extreme manifestation of such response is nascent peptide-dependent ribosome stalling, involved in the regulation of gene expression. The molecular mechanisms of programmed translation arrest are unclear. By analyzing ribosome stalling at the regulatory cistron of the antibiotic resistance gene ermA, we uncovered a carefully orchestrated cooperation between the ribosomal exit tunnel and the A-site of the peptidyl transferase center (PTC) in halting translation. The presence of an inducing antibiotic and a specific nascent peptide in the exit tunnel abrogate the ability of the PTC to catalyze peptide bond formation with a particular subset of amino acids. The extent of the conferred A-site selectivity is modulated by the C-terminal segment of the nascent peptide, where the third-from-last residue plays a critical role.

Graphical AbstractFigure optionsDownload high-quality image (276 K)Download as PowerPoint slideHighlights
► In the presence of erythromycin, the ribosome stalls at the 8th codon of ermAL1
► Stalled ribosome is unable to catalyze peptide bond formation with the 9th amino acid
► The nature of the A-site codon is critical for stalling
► Nascent peptide sequence renders the A-site selective to the nature of aminoacyl-tRNA