کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1996944 | 1065527 | 2010 | 11 صفحه PDF | دانلود رایگان |
SummaryProfound chromatin changes occur during mitosis to allow for gene silencing and chromosome segregation followed by reactivation of memorized transcription states in daughter cells. Using genome-wide sequencing, we found H2A.Z-containing +1 nucleosomes of active genes shift upstream to occupy TSSs during mitosis, significantly reducing nucleosome-depleted regions. Single-molecule analysis confirmed nucleosome shifting and demonstrated that mitotic shifting is specific to active genes that are silenced during mitosis and, thus, is not seen on promoters, which are silenced by methylation or mitotically expressed genes. Using the GRP78 promoter as a model, we found H3K4 trimethylation is also maintained while other indicators of active chromatin are lost and expression is decreased. These key changes provide a potential mechanism for rapid silencing and reactivation of genes during the cell cycle.
► Global H2A.Z patterns are maintained during mitosis
► +1 Nucleosomes shift during mitosis, reducing nucleosome-depleted regions
► +1 Nucleosome shifting during mitosis is restricted to transiently silenced promoters
Journal: - Volume 39, Issue 6, 24 September 2010, Pages 901–911