Imaging-Based Identification of a Critical Regulator of FtsZ Protofilament Curvature in Caulobacter

SummaryFtsZ is an essential bacterial GTPase that polymerizes at midcell, recruits the division machinery, and may generate constrictive forces necessary for cytokinesis. However, many of the mechanistic details underlying these functions are unknown. We sought to identify FtsZ-binding proteins that influence FtsZ function in Caulobacter crescentus. Here, we present a microscopy-based screen through which we discovered two FtsZ-binding proteins, FzlA and FzlC. FzlA is conserved in α-proteobacteria and was found to be functionally critical for cell division in Caulobacter. FzlA altered FtsZ structure both in vivo and in vitro, forming stable higher-order structures that were resistant to depolymerization by MipZ, a spatial determinant of FtsZ assembly. Electron microscopy revealed that FzlA organizes FtsZ protofilaments into striking helical bundles. The degree of curvature induced by FzlA depended on the nucleotide bound to FtsZ. Induction of FtsZ curvature by FzlA carries implications for regulating FtsZ function by modulating its superstructure.

► An imaging screen identifies uncharacterized FtsZ-binding proteins
► FzlA is critical for Caulobacter cell division and alters FtsZ structure in vivo
► Nucleotide-dependent helical bundles are formed by FtsZ and FzlA in vitro
► Induction of FtsZ curvature by FzlA represents a unique mode of FtsZ regulation