کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1997274 1065563 2008 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Respiratory Active Mitochondrial Supercomplexes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Respiratory Active Mitochondrial Supercomplexes
چکیده انگلیسی

SummaryThe structural organization of the mitochondrial respiratory complexes as four big independently moving entities connected by the mobile carriers CoQ and cytochrome c has been challenged recently. Blue native gel electrophoresis reveals the presence of high-molecular-weight bands containing several respiratory complexes and suggesting an in vivo assembly status of these structures (respirasomes). However, no functional evidence of the activity of supercomplexes as true respirasomes has been provided yet. We have observed that (1) supercomplexes are not formed when one of their component complexes is absent; (2) there is a temporal gap between the formation of the individual complexes and that of the supercomplexes; (3) some putative respirasomes contain CoQ and cytochrome c; (4) isolated respirasomes can transfer electrons from NADH to O2, that is, they respire. Therefore, we have demonstrated the existence of a functional respirasome and propose a structural organization model that accommodates these findings.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 32, Issue 4, 21 November 2008, Pages 529–539
نویسندگان
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