کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1997448 1065579 2009 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Global Map of SUMO Function Revealed by Protein-Protein Interaction and Genetic Networks
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Global Map of SUMO Function Revealed by Protein-Protein Interaction and Genetic Networks
چکیده انگلیسی
Systematic functional genomics approaches were used to map a network centered on the small ubiquitin-related modifier (SUMO) system. Over 250 physical interactions were identified using the SUMO protein as bait in affinity purification-mass spectrometry and yeast two-hybrid screens. More than 500 genes and 1400 synthetic genetic interactions were mapped by synthetic genetic array (SGA) analysis using eight different SUMO pathway query genes. The resultant global SUMO network highlights its role in 15 major biological processes and better defines functional relationships between the different components of the SUMO pathway. Using this information-rich resource, we have identified roles for the SUMO system in the function of the AAA ATPase Cdc48p, the regulation of lipid metabolism, localization of the ATP-dependent endonuclease Dna2p, and recovery from the DNA-damage checkpoint.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Cell - Volume 33, Issue 1, 16 January 2009, Pages 124-135
نویسندگان
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